Following a brief introduction to biological agents and the hazards generated by these agents, e-fact includes sections on ‘How to do a Risk Assessment’ and ‘How to use a Checklist’. A checklist is then presented to help identify the hazards potentially posed by biological agents. An extensive list of ‘proposed solutions and examples of preventive measures’ is then considered in the light of some of the questions raised in the general checklist. An example of risk assessment relating to legionella is then presented. Finally, sources of further information are presented at the end of the e-fact.